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p nf κb cell signaling technology 8242s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p nf κb cell signaling technology 8242s
    P Nf κb Cell Signaling Technology 8242s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 8774 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Article Title: Monocarbonyl analogs of curcumin C66 and B2BrBC modulate oxidative stress, JNK activity, and pancreatic gene expression in rats with streptozotocin-induced diabetes.
    Article Snippet: Antibody Source Catalog # Company Dilution JNK1 Mouse 3708 Cell Signaling Technology 1:1000 P-SAPK/JNK Mouse 9255 Cell Signaling Technology 1:1000 NF-κB Rabbit 8242 Cell Signaling Technology 1:1000 p-NF-κB Rabbit 3033 Cell Signaling Technology 1:1000 β-Actin Mouse 3700 Cell Signaling Technology 1:1000 INS Mouse 8138 Cell Signaling Technology 1:1000 GLUT2 Rabbit PA5-97263 Thermo Fisher Scientific 1:1000 anti-Rb IgG Goat 31,460 Thermo Fisher Scientific 1:5000 anti-Mo IgG Goat 31,430 Thermo Fisher Scientific 1:5000 R. Stojchevski et al. Biochemical Pharmacology 229 (2024) 116491 μM) dissolved in DMSO (Sigma-Aldrich, St. Louis, MO, USA) for 72 h, followed by STZ treatment (1.5 mM, dissolved in Na-citrate buffer, pH 4.5) for an additional 24 h.



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    FIGURE 6. Impact of β2-AR blockade on corneal inflammatory response. (A) Volcano plot depicting differentially expressed genes in dry eye mouse corneas with ICI 118,551 (ICI) or vehicle treatment (n = 4 per group). (B) Top 10 KEGG enrichment of downregulated genes in ICI versus vehicle-treated corneas. (C) Heatmap showing differentially expressed genes. (D) Comparison of Cxcl1, Cxcl2, IL17c, and S100a9 mRNA transcripts in mouse corneas (n = 5 per group). (E) CD45 staining of corneal cross-sections. Scale bar = 50 μm, n = 5 per group. (F) Nuclear translocation <t>of</t> <t>NF-κB</t> <t>p65</t> in normal medium, 500 mOsM medium, and 500 mOsM medium supplemented with ICI. Scale bar = 20 μm. (G) The protein levels of p-NF-κB p65 (n = 5 per group). (H) Comparison of TNF-α, IL-1β, CXCL1, and CXCL2 mRNA transcripts in HCECs (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant.
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    FIGURE 6. Impact of β2-AR blockade on corneal inflammatory response. (A) Volcano plot depicting differentially expressed genes in dry eye mouse corneas with ICI 118,551 (ICI) or vehicle treatment (n = 4 per group). (B) Top 10 KEGG enrichment of downregulated genes in ICI versus vehicle-treated corneas. (C) Heatmap showing differentially expressed genes. (D) Comparison of Cxcl1, Cxcl2, IL17c, and S100a9 mRNA transcripts in mouse corneas (n = 5 per group). (E) CD45 staining of corneal cross-sections. Scale bar = 50 μm, n = 5 per group. (F) Nuclear translocation <t>of</t> <t>NF-κB</t> <t>p65</t> in normal medium, 500 mOsM medium, and 500 mOsM medium supplemented with ICI. Scale bar = 20 μm. (G) The protein levels of p-NF-κB p65 (n = 5 per group). (H) Comparison of TNF-α, IL-1β, CXCL1, and CXCL2 mRNA transcripts in HCECs (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant.
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    FIGURE 6. Impact of β2-AR blockade on corneal inflammatory response. (A) Volcano plot depicting differentially expressed genes in dry eye mouse corneas with ICI 118,551 (ICI) or vehicle treatment (n = 4 per group). (B) Top 10 KEGG enrichment of downregulated genes in ICI versus vehicle-treated corneas. (C) Heatmap showing differentially expressed genes. (D) Comparison of Cxcl1, Cxcl2, IL17c, and S100a9 mRNA transcripts in mouse corneas (n = 5 per group). (E) CD45 staining of corneal cross-sections. Scale bar = 50 μm, n = 5 per group. (F) Nuclear translocation <t>of</t> <t>NF-κB</t> <t>p65</t> in normal medium, 500 mOsM medium, and 500 mOsM medium supplemented with ICI. Scale bar = 20 μm. (G) The protein levels of p-NF-κB p65 (n = 5 per group). (H) Comparison of TNF-α, IL-1β, CXCL1, and CXCL2 mRNA transcripts in HCECs (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant.
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    FIGURE 6. Impact of β2-AR blockade on corneal inflammatory response. (A) Volcano plot depicting differentially expressed genes in dry eye mouse corneas with ICI 118,551 (ICI) or vehicle treatment (n = 4 per group). (B) Top 10 KEGG enrichment of downregulated genes in ICI versus vehicle-treated corneas. (C) Heatmap showing differentially expressed genes. (D) Comparison of Cxcl1, Cxcl2, IL17c, and S100a9 mRNA transcripts in mouse corneas (n = 5 per group). (E) CD45 staining of corneal cross-sections. Scale bar = 50 μm, n = 5 per group. (F) Nuclear translocation of NF-κB p65 in normal medium, 500 mOsM medium, and 500 mOsM medium supplemented with ICI. Scale bar = 20 μm. (G) The protein levels of p-NF-κB p65 (n = 5 per group). (H) Comparison of TNF-α, IL-1β, CXCL1, and CXCL2 mRNA transcripts in HCECs (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant.

    Journal: Investigative ophthalmology & visual science

    Article Title: Activation of Sympathetic Nervous System Drives Dry Eye Onset Via Norepinephrine-β2-Adrenergic Receptor Signaling in Mice.

    doi: 10.1167/iovs.66.6.13

    Figure Lengend Snippet: FIGURE 6. Impact of β2-AR blockade on corneal inflammatory response. (A) Volcano plot depicting differentially expressed genes in dry eye mouse corneas with ICI 118,551 (ICI) or vehicle treatment (n = 4 per group). (B) Top 10 KEGG enrichment of downregulated genes in ICI versus vehicle-treated corneas. (C) Heatmap showing differentially expressed genes. (D) Comparison of Cxcl1, Cxcl2, IL17c, and S100a9 mRNA transcripts in mouse corneas (n = 5 per group). (E) CD45 staining of corneal cross-sections. Scale bar = 50 μm, n = 5 per group. (F) Nuclear translocation of NF-κB p65 in normal medium, 500 mOsM medium, and 500 mOsM medium supplemented with ICI. Scale bar = 20 μm. (G) The protein levels of p-NF-κB p65 (n = 5 per group). (H) Comparison of TNF-α, IL-1β, CXCL1, and CXCL2 mRNA transcripts in HCECs (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant.

    Article Snippet: TH Merck-Millipore AB1542 IF:1:200 c-FOS Abcam ab190289 IF:1:200 NE Sigma-Aldrich AB120 IF:1:200 MMP3 Abcam ab73955 IF:1:200 MMP9 Proteintech 10375-2-AP IF:1:200 TUBB3 Merck-Millipore 15708A4 IF:1:200 CD45 Servicebio GB113886 IF:1:200 NF-κB p65 Servicebio GB11997 IF:1:200 TNF-α Servicebio GB115702 IHC:1:200 IL-1β Servicebio GB11113 IHC:1:200 F4/80 Servicebio GB113373 IHC:1:200 NF-κB p65 Cell Signaling Technology 8242 WB:1:500 Phospho-NF-κB p65 Cell Signaling Technology 3033 WB:1:500 IF, immunofluorescence; IHC, immunohistochemistry; WB, Western blotting.

    Techniques: Comparison, Staining, Translocation Assay